T7 RNA Polymerase ELISA Kit
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with high specificity for the T7 promoter. This enzyme catalyzes the 5’→3’ synthesis of RNA from DNA downstream from its promoter.
Croyez ® T7 RNA Polymerase ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of T7 RNA Polymerase level in sample solution. The T7 RNA Polymerase ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.
Croyez ® T7 RNA Polymerase ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of T7 RNA Polymerase level in sample solution. The T7 RNA Polymerase ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.
Method:
Sandwich ELISA
Principle of Assay:
T7 RNA Polymerase ELISA kit is used to detect T7 RNA Polymerase in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-T7 RNA Polymerase monoclonal antibody to the solid phase. T7 RNA Polymerase in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-T7 RNA Polymerase monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of T7 RNA Polymerase protein level is accomplished by comparing the absorbance with standard curve.
Platform:
Pre-coated 96 Microplate (12 x 8 well strips)
Sensitivity:
Limit of detection (LoD): 0.037 ng/mL
Limit of quantification (LoQ): 0.124 ng/mL.
Detection Range:
0.15625-10 ng/mL
Reactivity:
T7 RNA Polymerase
Components:
T7 RNA Polymerase ELISA plate
Standard
Standard reconstitution buffer
Standard & Sample diluent buffer
HRP-antibody conjugate
HRP-antibody conjugated diluent buffer
20 X wash buffer
TMB
Stop solution
Microplate sealing film
Storage:
Store at 2-8°C.
Usage:
For Research Use Only
Shipping Conditions:
Blue ice
Sandwich ELISA
Principle of Assay:
T7 RNA Polymerase ELISA kit is used to detect T7 RNA Polymerase in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-T7 RNA Polymerase monoclonal antibody to the solid phase. T7 RNA Polymerase in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-T7 RNA Polymerase monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of T7 RNA Polymerase protein level is accomplished by comparing the absorbance with standard curve.
Platform:
Pre-coated 96 Microplate (12 x 8 well strips)
Sensitivity:
Limit of detection (LoD): 0.037 ng/mL
Limit of quantification (LoQ): 0.124 ng/mL.
Detection Range:
0.15625-10 ng/mL
Reactivity:
T7 RNA Polymerase
Components:
T7 RNA Polymerase ELISA plate
Standard
Standard reconstitution buffer
Standard & Sample diluent buffer
HRP-antibody conjugate
HRP-antibody conjugated diluent buffer
20 X wash buffer
TMB
Stop solution
Microplate sealing film
Storage:
Store at 2-8°C.
Usage:
For Research Use Only
Shipping Conditions:
Blue ice